Electroimmunoassay. A new competitive protein-binding assay using antibody-sensitive electrodes

Competitive protein-binding assay for 25-hydroxycholecalciferol.

1. A competitive protein-binding assay for plasma 25-hydroxycholecalciferol was developed, using Sephadex LH-20 columns for the isolation of the sterol and a partially purified protein from rat serum as the binding protein. 2. The mean plasma 25-hydroxycholecalciferol in eighteen male and female normal volunteers was 38 5 SD 14 pmol/ml (1 5.2 & 5.6 nglml). 3. Levels of 25-hydroxycholecalciferol...

Development of a Sensitive Quantitative Competitive PCR Assay for Detection of Human Cytomegalovirus DNA

Accurate and rapid diagnosis of human cytomegalovirus (HCMV) disease in immunocompromised patients has remained as a challenge. Quantitative competitive PCR (QC-PCR) methods for detection of HCMV in these individuals have improved the positive and negative predictive values of PCR for diagnosis of HCMV disease. In this study we used QC-PCR assay, using a co-amplified DNA standard, to quantitate...

Rapid, specific assay for plasma cortisol by competitive protein binding.

We describe a modified competitive protein-binding method for assay of plasma cortisol. Plasma samples are deproteinized by dilution with an ethanol/phosphate buffer, followed by heating at 100 degrees C for 2 min. Horse serum is used as the source of transcortin. Free radioactivity is separated from the protein-bound component by partition into liquid scintillation counting within 60 min. The ...

A Competitive-Binding Assay forGlycopeptideAntibioticsof the VancomycinClass

A solid-phase enzyme-receptor assay (sPcR.A) has been developed for glycopeptide antibiotics of the vancomycin class such as teicoplanin, vancomycin, ristocetin, avoparcin, actaplanin,A-47934, A-41 030, and A-3551 2-B. The assay exploitsthe mechanismof most action of these antibiotics, which is based on their interaction with acyl-D-alanyl-Dalanine, a constituentof the walls of mostgrowingbacte...

Enzyme-Linked Immunosorbent Assay Competitive Binding Inhibition